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KMID : 0363320190400040567
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Journal of Korean Oriental Internal Medicine
2019 Volume.40 No. 4 p.567 ~ p.581
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Inhibitory Effects of SGX01 on Lung Injury of COPD Mice Model
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Park Jae-Jun
Yang Won-Kyung
Lyu Yee-Ran
Kim Seung-Hyung
Park Yang-Chun
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Abstract
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Objective: This study aimed to evaluate the inhibitory effects of SGX01 on the lung injuries of COPD mice model. Materials and
Methods: This study was carried out in two ways: in vitro and in vivo. In vitro, L929 cells were challenged with LPS, and then treated with six concentrations of SGX01 (10, 30, 50, 100, 300, and 500 μg/ml) and analyzed by ELISA. In vivo, C57BL/6 mice were challenged with LPS and cigarette smoking solution (CSS), and then treated with a vehicle only (control group), dexamethasone 3 mg/kg (dexa group), or a SGX01 200 mg/kg (SGX01 group). After sacrifice, the BALF or lung tissue was analyzed with Cytospin, FACS, ELISA, real-time PCR and H&E, and Masson’s trichrome staining.
Results: SGX01 significantly decreased NO, TNF-α, and IL-6 on L929 cells challenged with LPS. In the COPD model, SGX01 significantly inhibited the increase of neutrophils, TNF-α, IL-17A, CXCL-1, MIP2, CD8+ cells in BALF, and TNF-α, IL-1β mRNA expression in lung tissue. It also decreased the severity of the histological lung injury.
Conclusion: This study suggests the usability of SGX01 for COPD patients by controlling lung tissue injury.
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KEYWORD
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SGX01, chronic obstructive pulmonary disease, cigarette smoke solution
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